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Penttilä H, M.D. (1), Huttunen P, Ph.D.(2),
von Smitten K, M.D., Ph.D. (3), Waris T, M.D., Ph.D.(4,5,6), Ashammakhi
N, M.D., Ph.D., F.R.C.S.Ed.(6,7).
1. Department of Oral and Maxillofacial Diseases, Surgical
Hospital, Helsinki University Central Hospital,
Helsinki, Finland
2. Department of Forensic Medicine, University of Oulu, Oulu, Finland
3. Maria Hospital, Helsinki, Finland
4. Medical School, University of Tampere, Tampere, Finland
5. Division of Plastic Surgery, Department of Surgery, Tampere University Hospital,
Tampere, Finland
6. Division of Plastic Surgery, Department of Surgery, Oulu University Hospital,
Oulu, Finland
7. Institute of Biomaterials, Tampere University of Technology, Tampere, Finland
Abstract:
The aim of this study was to investigate the rate of disappearance of
noradrenaline in arterial grafts after their transplantation. Arterial
grafts were harvested from AO/Ks:OC strain rats. In each rat, a graft
was harvested from the femoral artery and transplanted into the common
carotid artery. The contralateral non-operated femoral artery served
as control. High performance liquid chromatography (HPLC) with electrochemical
detection was used to determine the noradrenaline content in the grafts
and controls. Immediately after grafting the noradrenaline content
was 76% of control, at 6h it was 130% and it then decreased to 4% of
the control value at 48h after grafting. The drop in graft noradrenaline
content (from 0h to 48h) was statistically significant (p=0.005). The
difference between 0h, 12h and 24h groups was statistically insignificant.
Compared with control specimens, the grafts contained less noradrenaline
after 12h and it disappeared almost completely within 48 hours.
Keywords:
Arterial grafts - denervation – noradrenaline – HPLC – experimental surgery
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