Viral Gene Transfer

CRISPR Feasibility

• Feasibility of a cell line for CRISPR gene knockout modification by lentiviral transduction
• The service can be made for mycoplasma-tested cell lines or for primary cells
• The cells are infected with GFP lentivirus and the resulting transduction efficiency is determined
• The customer receives information if their target cell line is feasible for lentiCRISPR approach
• The service price is 50 €
• The service is delivered in 2-3 days after receiving the cells

CRISPR Knockout

• Preparation of a CRISPR gene modified cell line with lentivirus infection
• The service requires CRISPR Feasibility information
• The service includes design and cloning of gRNA into lentiviral CRISPR-vector, infection of target cells and selection of positively transduced cell population
• The customer receives CRISPR knockout cell population
• The service price is 3000 €
• The service is delivered in 2 months after CRISPR Feasibility information

Lentivirus vector

• Generation of recombinant lentiviral particles from lentiviral expression plasmid construct (provided by the customer)
• Viral vectors can be concentrated by ultracentrifugation
• The customer receives lentivirus vector (0.5 ml for concentrated preps or 5-20 ml for unconcentrated preps)
• The service price is 500 €
• The service is delivered within 2 weeks after receiving the plasmid
• Additional services:
  • Purification of viral vectors by anion exchange HPLC, according to separate offer
  • Plasmid production 200 € (plasmid map available and only common enzymes needed)
  • Virus content by titration 110 €
• For electronic service order forms contact VirusCore staff

Retrovirus vector

• Generation of recombinant murine retroviral particles from retroviral expression plasmid construct (provided by the customer)
• Virus vector can be concentrated and purified using ultracentrifugation
• The customer receives retroviral vector (0.5 ml for concentrated preps or 5-20 ml for unconcentrated preps)
• The service price is 500 €
• The service is delivered within 2 weeks after receiving of the plasmid
• Additional services:
  • Purification of viral vectors by anion exchange HPLC, according to separate offer
  • Plasmid production 200 € (plasmid map available and only common enzymes needed)
  • Virus content by titration 110 €
• For electronic service order forms contact VirusCore staff

Adenovirus vector

• Transfection of a linearized adenoviral expression plasmid (provided by the customer) into adenoviral packaging system and generation of recombinant adenoviral vector
• Viral vector is purified by Optiprep gradient isolation and viral content is determined by photometric measurement
• The customer receives adenovirus vector
• The service price is 500 €
• The service is delivered within 2-3 weeks after delivery of the plasmid
• Additional services:
  • Chromatographic purification available according to separate offer
  • Additional production of adenovirus vector from existing viral prep 250 € (adeno-expansion)
  • Cloning service for adenoviral vector 500 €
• For electronic service order forms contact VirusCore staff