Basic Course on Light and Electron Microscopy

Time:

Feb 1st to Feb 3rd, 2023

Place:

BCO Light Microscopy (LM) and Electron Microscopy (EM) Core Facility Laboratories, the Main Building of the Medical Campus, University of Oulu, Aapistie 5A, 90220 Oulu, Finland

Credits:

1 ETCS

Organisers:

Health and Biosciences Doctoral Programme and Oulu BioImaging, Biocenter Oulu, University of Oulu, https://www.oulu.fi/en/university/faculties-and-units/biocenter-oulu

Aim:

To get acquainted with light and electron microscopy imaging methods of living and fixed specimens of different sizes. Learn how to apply suitable techniques for image analysis. LM introduction includes widefield and confocal fluorescence microscopy and examples of multiphoton techniques. EM part focuses on sample preparation and transmission and scanning electron microscopy. The course consists of lectures, microscopy demonstrations and computer-based image analysis workshops.

Information:

Veli-Pekka Ronkainen / Ilkka Miinalainen, Biocenter Oulu, tel: +358 294 486114 / +358 294 486145, e-mail: veli-pekka.ronkainen(at)oulu.fi / ilkka.miinalainen(at)oulu.fi

Registration:

Lectures are open for all without registration. Registration for the demonstrations is obligatory and preference will be given to Ph.D. students and researchers whose project benefits the topics carried out during the course. Due to space limitations only 18 participants can be accepted. The application with a short description of the applicant’s project should be submitted to Webropol by 25th of January 2023. Selected applicants will be informed by Jan 26th.

PROGRAMME

Wednesday, Feb 1st, 2023

Lecture hall P107 (Pathology)

Lectures:

9.00-9.05

Opening words and introduction

9.05-10.00

Basics in light/fluorescence microscopy, Veli-Pekka Ronkainen, BCO

10.00-10.15

Coffee break

10.15-11.15

Basics in electron microscopy, Ilkka Miinalainen, BCO

11.15-12.15

Lunch break

12.15-13.15

Principles of advanced fluorescence microscopy techniques, Veli-Pekka Ronkainen

13.15-13.30

Break

13.30-14.30

Methods for preparing biological samples for electron microscopy analysis, Ilkka Miinalainen

14.30-14.45

Introduction to demonstrations, Veli-Pekka Ronkainen

Thursday, Feb 2nd, 2023

8.45

Gathering in meeting room 487B (Kieppi Building 4th floor, Aapistie 5A) for demonstrations.

9.00-16.00

Demonstrations, 3 groups

• Demo 1. Laser scanning confocal and multiphoton microscopy, Room 423A and 492B. Imaging of cells and 3D imaging of thick mesoscopic samples, Veli-Pekka Ronkainen
• Demo 2. Transmission electron microscopy and sample preparation, Room 467B and 488B. Imaging of tissue samples and negative staining and imaging of macromolecules, Mika Kaakinen
• Demo 3. Scanning electron microscopy and sample preparation, Room 320A and 488B. Imaging of tissue and cell samples using different detectors and preparing samples for sputter coating, Ilkka Miinalainen

Hours 1st group 2nd group 3rd group

09.00-11.00 Demonstration 1 Demonstration 2 Demonstration 3

11.00-12.00 Lunch break

12.00-14.00 Demonstration 2 Demonstration 3 Demonstration 1

14.00-16.00 Demonstration 3 Demonstration 1 Demonstration 2

Friday, Feb 3rd, 2023

Room 289B and Computer Class KT285

9.00-10.00

Introduction to computer-based image processing and analysis,

Room 289B, Mika Kaakinen, BCO

10.00-10.15

Coffee break

10.15-11.45

Computer-based processing and analysis of light microscopy images,

Computer Class KT285, Veli-Pekka Ronkainen, Antti Viklund, Mika Kaakinen

11.45-12.45

Lunch break

12.45-14.15

Computer-based processing and analysis of electron microscopy images,

Computer Class KT285, Ilkka Miinalainen, Antti Viklund, Mika Kaakinen

14.15

Concluding remarks