Saara Koivusalo

I am applying digital holographic microscopy (DHM) to image organoids. In DHM light from a coherent laser illuminates the sample which changes the phase of the light. When compared to referense beam, these two beams are combined to form a hologram which is captured by camera and interpreted by the computer. This allowes a real-time imaging and quantitative measurements of morphological parameters without any staining or labeling.

By using DHM we are analyzing the morphology of prostate cancer organoids in relation to pathology. The quantitative phase information from DHM may accurately classify cancer cell subpopulations with clinical relevance. A future goal is to make the morphological analysis ability of DHM as a fast, automatic, and cost efficient tool for testing different cancer treatments.