Novel NMR methods applied to biochemical systems

In this doctoral thesis, a group of novel NMR spectroscopy methods was developed for the study of biochemical systems. NMR spectroscopy is a technique that allows for the investigation of the structures, interactions, and movements of molecules. It is particularly advantageous for studying biological samples because biomolecules can be investigated under physiological conditions, and since the method relies on the use of radiofrequency radiation, it does not damage the sample being studied at all. Medical magnetic resonance imaging used in hospitals is one of the well-known applications of NMR spectroscopy.

The NMR methods presented in the dissertation provide new tools for studying pore structure of biomaterials. Research on pore structure is important because it is closely related to the progression of degenerative diseases, such as osteoarthritis. In addition, this thesis introduced new rapid relaxation experiments that can be used to study dynamic processes of biomolecules and identify different molecular environments present in the sample. Accelerated methods are highly beneficial in the study of biological samples, as these samples can change or degrade during prolonged measurements. Fast measurement techniques also enable the enhancement of signal of low-concentration substances via hyperpolarization techniques, which can be necessary, for example, in the study of metabolic products.